Organ(elle) Transplants

Organelle proteomics.

This unit describes strategies for studying the proteomes of organelles, which is one example of targeted proteomics. It relies heavily on previously published units dealing with organelle preparation, protein solubilization, and proteomics techniques. A specific commentary for organelle proteomics is provided. Specific protocols for the isolation of nuclei from various sources (cell cultures, ...

Organelle Inheritance

The need to inherit those membranes that arise from the ER is much less clear. As an example, the integral *Imperial Cancer Research Fund Cell Biology Laboratory membrane proteins and lipids of the Golgi apparatus are derived entirely from the ER. If a daughter did not 44 Lincoln's Inn Fields London WC2A 3PX inherit a copy of the Golgi apparatus, it should be able to synthesize one de novo. Sev...

Cellular transplants.

Faecal transplants.

Recurrent Clostridium difficile infection is a severe and often fatal condition, affecting up to 3000 people in the United Kingdom and 100 000 in the United States, where it kills an estimated 14 000 a year.About a quarter of patients experience a recurrence after an initial mild infection because treatment with antibiotics destroys the diversity of the normal gut microbes and allows C difficil...

Mechanisms of organelle biogenesis govern stochastic fluctuations in organelle abundance

Fluctuations in organelle abundance can profoundly limit the precision of cell biological processes from secretion to metabolism. We modeled the dynamics of organelle biogenesis and predicted that organelle abundance fluctuations depend strongly on the specific mechanisms that increase or decrease the number of a given organelle. Our model exactly predicts the size of experimentally measured Go...